Physical State: Liquid
This product is a specially formularized IFN-¦Ã Cell Stimulant solution. It is in an easy-to-use format. Interferon-gamma (IFN-¦Ã) originally called macrophage-activating factor is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-¦Â or the various IFN-¦Á family proteins. Mature IFN-¦Ã exists as homodimers. Human IFN-¦Ã is highly species specific and is biologically active only in human and primate cells. IFN-¦Ã has antiviral, immunoregulatory, and anti-tumour properties. The protein also has the following activities: Increase antigen presentation of macrophages; Activate and increase lysosome activity in macrophages; Suppress Th2 cell activity; Cause normal cells to express class I MHC molecules; Promotes adhesion and binding required for leukocyte migration; Promotes NK cell activity. IFN-¦Ã exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-¦Ã receptor is present on almost all cell types except mature erythrocytes. The IFN-¦Ã receptor is structurally related to the recently cloned IL-10 receptor.
1. Mix 200l of 50X Cell Stimulant solution with 9800l of medium (for one 96-well plate). This diluted mixture must be used within the day. Note: Although the product is sterilized, user may need to filter the diluted solution with 0.2 filter to prevent any contamination during the dilution process.
2. Apply 100l of the above mixture to a well (96-well plate) and incubate cells at 37¡æ / 5%CO2 for 4hr~16hr. Collect the medium for further assays.
Storage Conditions: Store container at -20¡æ for six months or at -70¡æ for one year from date of receipt. Distilled 1X mixture must be used within one day.
Note: This product is for research use only and is not intended for therapeutic or diagnostic applications.