Ready-to-Use™ human IL-1b Cell Stimulant (for R&D use only)

Ready-to-Use™ human IL-1b Cell Stimulant
Item# CS-103
Regular price: $50.00
Sale price: $46.00

Product Description

Physical State: Liquid

Description: This product is a specially formularized IL-1b Cell Stimulant solution. It is in an easy-to-use format. IL-1 and IL-1 are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 and IL-1 are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 or IL-1 and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 but functions as a decoy receptor and negative regulator of IL-1 activity. IL-1ra functions as a competitive antagonist by preventing IL-1 and IL-1 from interacting with IL-1 RI. The human IL-1 cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1-converting enzyme (Caspase-1/ICE) to generate the active cytokine.

Application Instruction:

1. Mix 200l of 50X IL-1b Cell Stimulant solution with 9800l of medium (for one 96-well plate). This diluted mixture must be used within the day. Note: Although the product is sterilized, user may need to filter the diluted solution with 0.2 filter to prevent any contamination during the dilution process.

2. Apply 100l of the above mixture to a well (96-well plate) and incubate cells at 37 / 5%CO2 for 4hr~16hr. Collect the medium for further assays.

Storage Conditions: Store container at -20 for six months or at -70 for one year from date of receipt. Distilled 1X mixture must be used within one day.

Note: This product is for research use only and is not intended for therapeutic or diagnostic applications.