Physical State: Liquid
Description: This product is a specially formularized TNF-¦Á Cell Stimulant solution. It is in an easy-to-use format. TNF-¦Á regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-¦Á is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.
1. Mix 200l of 50X TNF-¦Á Cell Stimulant solution with 9800l of medium (for one 96-well plate). . This diluted mixture must be used within the day. Note: Although the product is sterilized, user may need to filter the diluted solution with 0.2 filter to prevent any contamination during the dilution process.
2. Apply 100l of the above mixture to a well (96-well plate) and incubate cells at 37¡æ / 5%CO2 for 4hr~16hr. Collect the medium for further assays.
Storage Conditions: Store container at -20¡æ for six months or at -70¡æ for one year from date of receipt. Distilled 1X mixture must be used within one day.
Note: This product is for research use only and is not intended for therapeutic or diagnostic applications.